![]() ![]() Do not check Global unless you want all your images to have this calibration.Enter the dimensions of the object/scale bar in the “ known distance” box and set the units in the " Unit of length" box.Run the menu command “ Analyze/Set scale…”.Do not choose " Edit/Draw" after adding the line. Using the line selection tool, draw a line along the length of the feature or scale bar.If you know the size of a feature (for example, a previously applied scale bar) you can use this command to apply a calibration. In the new dialog, enter a value by which the receiving image has been scaled.In the “ from" drop-down box select the original image in the “ to" box, select the processed image.Open the image with the original calibration.There are three ways to reapply spatial calibration: manually via the “ Image/Properties” dialog using the "Copy Pixel Size" plugin that applies pixel size of one image to a second image or with the "Set Scale" plugin.Įrror creating thumbnail: Unable to save thumbnail to destination In this case, use the original file's spatial calibration (located under " Image/Properties") to update the " Image/Properties" values of the final, processed image. Sometimes the spatial calibration may be lost after applying an image processing function. The " Microscope Scale" plugin is another alternative and can be customized using a drop-down box of objectives to apply spatial calibrations. Spatial calibration can also be found and set in the " Image/Properties" dialog. Then the calibration can be applied using " Analyze/Set Scale" (see below). These settings include camera binning, frame size, objective, confocal zoom, etc. In the case that the files have no calibration, you can apply the calibration using images of a stage micrometer with the same settings as your experiment. Zeiss zvi's exported as TIFFs) will lose their calibration information completely. Perkin Elmer) are not calibrated while some "exported" image files (e.g. However, it is important to keep in mind that some file formats (e.g. Some microscope models (e.g., Biorad PIC, and Zeiss LSM confocal image files) will use the zoom and objective magnification settings for internal calibration. Note that the units "um" will automatically change to “µm”. A standard size should be used for the scale bars on all images if possible to help avoid confusion.Īn image's spatial calibration can be edited using " Image/Properties". All images for publications should include a scale bar. ![]()
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